anti phosphor stat6tyr641  (Cell Signaling Technology Inc)

Journal: Immune Network

Article Title: STING-STAT6 Signaling Pathway Promotes IL-4 + and IFN-α + Fibrotic T Cell Activation and Exacerbates Scleroderma in SKG Mice

doi: 10.4110/in.2024.24.e37

Figure Lengend Snippet: WT, wild type; STAT6 i, STAT6 inhibitor. * p<0.05, ** p<0.01, and *** p<0.001.

Article Snippet: We used anti-CD4 (Novus Biologicals, Centennial, CO, USA), anti-IL-4 (Invitrogen), anti-IL-17 (Invitrogen), anti-IFNα (Invitrogen), anti-phosphor STING (Invitrogen), anti-phosphor IRF3 (Cell Signaling), anti-phosphor STAT6 (Invitrogen), anti-GM130 (Santa Cruz Biotechnology), anti-STING (Cell Signaling Technology), anti-STIM1 (Invitrogen), anti-ORAI1 (Invitrogen) Abs, α-SMA and fibronectin (Abcam, Cambridge, UK).

Techniques:

Journal: Immune Network

Article Title: STING-STAT6 Signaling Pathway Promotes IL-4 + and IFN-α + Fibrotic T Cell Activation and Exacerbates Scleroderma in SKG Mice

doi: 10.4110/in.2024.24.e37

Figure Lengend Snippet: WT, wild type; STAT6 i, STAT6 inhibitor. * p<0.05, ** p<0.01, and *** p<0.001.

Article Snippet: Then these cells were permeabilized using Cytofix/Cytoperm solution (BD Pharmingen, Franklin Lakes, NJ, USA), and subjected to intracellular staining using PE-conjugated anti-IL-4 (BD Biosciences), APC-conjugated anti-IL-17 (eBioscience), FITC-conjugated anti-IFNα (R&D Systems Minneapolis, MN, USA), anti-phosphor STING (Invitrogen, Waltham, MA, USA), anti-phosphor IRF3 (Cell Signaling Technology, Danvers, MA, USA), and anti-phosphor STAT6 (Santa Cruz Biotechnology, Dallas, TX, USA) Abs.

Techniques: